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imikhiqizo

Amafutha ahlanzekile e-Artemisia capillaris ekhandlela nensipho enza i-wholesale diffuser uwoyela obalulekile ube masha emishini yokushisa umhlanga

incazelo emfushane:

Idizayini yemodeli yegundane

Izilwane zahlukaniswa ngokungahleliwe zaba amaqembu amahlanu amagundane ayishumi nanhlanu ngalinye. Iqembu elilawulayo kanye namagundane eqembu eliyimodeli aye agavaged ngawoamafutha e-sesameizinsuku ezingu-6. Amagundane eqembu elilawulayo afakwe amaphilisi e-bifendate (BT, 10 mg/kg) izinsuku ezingu-6. Amaqembu okuhlola aphathwe nge-100 mg/kg kanye ne-50 mg/kg AEO encibilikiswe emafutheni esisame izinsuku ezingu-6. Ngosuku lwe-6, iqembu lokulawula laphathwa ngamafutha e-sesame, futhi wonke amanye amaqembu aphathwa ngomthamo owodwa we-0.2% CCl4 kuwoyela wesesame (10 ml/kg)umjovo we-intraperitoneal. Amagundane abe esezila ukudla ngaphandle kwamanzi, futhi amasampula egazi aqoqwa emikhunjini ye-retrobulbar; igazi eliqoqiwe laliyi-centrifuged ku-3000 ×gimizuzu eyi-10 ukuhlukanisa i-serum.Ukukhishwa komlomo wesibelethokwenziwa ngokushesha ngemva kokuhoxiswa kwegazi, futhi amasampula esibindi akhishwa ngokushesha. Ingxenye eyodwa yesampula yesibindi yagcinwa ngokushesha ku-−20 °C kuze kube yilapho ihlaziywa, futhi enye ingxenye yasikwa futhi yalungiswa ngo-10%formalinisisombululo; izicubu ezisele zagcinwa ku-−80 °C ukuze kuhlaziywe i-histopathological (Wang et al., 2008,Hsu et al., 2009,Nie et al., 2015).

Ukulinganisa amapharamitha we-biochemical ku-serum

Ukulimala kwesibindi kwahlolwa ngokulinganisaimisebenzi enzymaticye-serum ALT kanye ne-AST kusetshenziswa amakhithi okuhweba ahambisanayo ngokuya ngemiyalelo yekhithi (Nanjing, Isifundazwe saseJiangsu, eChina). Imisebenzi ye-enzymatic yavezwa njengamayunithi ilitha ngalinye (U/l).

Ukukalwa kwe-MDA, SOD, GSH kanye ne-GSH-Pxkuma-homogenates esibindi

Izicubu zesibindi zenziwa i-homogenized ne-saline ye-physiological ebandayo ngesilinganiso esingu-1:9 (w/v, isibindi:saline). Ama-homogenates ayengama-centrifuged (2500 ×gimizuzu eyi-10) ukuqoqa amandla amakhulu ukuze uthole izinqumo ezilandelayo. Ukulimala kwesibindi kwahlolwa ngokwezilinganiso ze-hepatic ze-MDA kanye namazinga e-GSH kanye ne-SOD ne-GSH-Pximisebenzi. Konke lokhu kwanqunywa ngokulandela imiyalelo yekhithi (eNanjing, eSifundazweni saseJiangsu, eChina). Imiphumela ye-MDA ne-GSH yavezwa njenge-nmol nge-mg protein (nmol/mg prot), kanye nemisebenzi ye-SOD ne-GSH-Pxzavezwa njenge-U per mg protein (U/mg prot).

Ukuhlaziywa kwe-Histopathological

Izingxenye zesibindi ezisanda kutholwa zalungiswa ku-10% ogcinwe ku-bufferparaformaldehydeisixazululo se-phosphate. Isampula libe selishumekwa kupharafini, lisikwe libe izingxenye ezi-3-5 μm, ezigcotshwei-hematoxylinfuthieosin(H&E) ngokuya ngenqubo ejwayelekile, futhi ekugcineni ihlaziywe ngui-microscope elula(Tian et al., 2012).

Ukuhlaziywa kwezibalo

Imiphumela yavezwa njengokuchezuka okujwayelekile okungu-± (SD). Imiphumela yahlaziywa kusetshenziswa uhlelo lwezibalo lwe-SPSS Statistics, inguqulo 19.0. Idatha ibe ngaphansi kokuhlaziywa kokuhluka (ANOVA,p< 0.05) okulandelwa ukuhlolwa kuka-Dunnett kanye nokuhlolwa kwe-T3 kuka-Dunnett ukuze kutholwe umehluko obalulekile ngokwezibalo phakathi kwamanani amaqembu okuhlola ahlukahlukene. Umehluko omkhulu ucatshangelwe ezingeni lep<0.05.

Imiphumela nengxoxo

Izingxenye ze-AEO

Ekuhlaziyweni kwe-GC/MS, i-AEO itholwe iqukethe izingxenye ze-25 ezikhishwe ku-10 kuya ku-35 min, futhi izakhi ze-21 ezibalelwa ku-84% wamafutha abalulekile zihlonziwe.Ithebula 1). Amafutha aguquguqukayo aqukethweama-monoterpenoids(80.9%), ama-sesquiterpenoids (9.5%), ama-hydrocarbons agcwele amagatsha (4.86%) kanye ne-miscellaneous acetylene (4.86%). Uma kuqhathaniswa nezinye izifundo (Guo et al., 2004), sithole ama-monoterpenoids amaningi (80.90%) ku-AEO. Imiphumela yabonisa ukuthi ingxenye egcwele kakhulu ye-AEO yi-β-citronellol (16.23%). Ezinye izingxenye ezinkulu ze-AEO zifaka i-1,8-cineole (13.9%),i-camphor(12.59%),linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),i-thymol(3.22%), kanyemyrcene(2.02%). Ukwehluka kokwakheka kwamakhemikhali kungase kuhlobane nezimo zemvelo isitshalo esachayeka kuzo, njengamanzi amaminerali, ukukhanya kwelanga, isigaba sokukhula kanyeumsoco.


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  • Imininingwane Yomkhiqizo

    Omaka bomkhiqizo

    Isifo sesibindi, ukuphazamiseka okuvamile okubangelwai-viral hepatitis, uphuzo oludakayo, amakhemikhali anobuthi esibindini, imikhuba yokudla engenampilo kanye nokungcoliswa kwemvelo, kuyinkinga yomhlaba wonke (UPapay et al., 2009). Nokho, ukwelashwa kwalesi sifo ngokuvamile kunzima ukuphatha futhi kunomphumela olinganiselwe. IsiShayina Sendabukoimithi ye-herbal, engaphansi kwemiyalelo eminingi esetshenziselwa ukwelapha izifo zesibindi, isasetshenziswa kabanzi amaShayina (UZhao et al., 2014).I-Artemisia capillarisThunb.,I-Asteraceae, ngokusho kwe-Bencao Gangmu, amarekhodi adume kakhulu e-Chinese Traditional Medicine, asetshenziswe kabanzi njengomuthi wokuqeda ukushisa, ukukhuthaza.i-diuresisfuthi isuse i-jaundice futhi iphinde yasetshenziswa njenge-flavour eziphuzweni, imifino, namakhekhe ngenxa yephunga layo elithile.A. capillarisiye yabhekwa njengohlobo lwemithi yesintu yaseShayina nokudla ngenani elandayo labantu. Ngakho-ke, kuye kwaba nemizamo emikhulu yokuthuthukisa imithi yamakhambi ewusizo, njengeA. capillaris, ukwelapha isifo sesibindi.

    Eminyakeni yamuva nje, imithi ye-herbal iye yathola ukunakwa okwengeziwe nokuthandwa kakhulu ekwelapheni isifo sesibindi ngenxa yokuphepha nokusebenza kwayo (Ding et al., 2012).A. capillariskufakazelwe ukuthi inomsebenzi omuhle we-hepatoprotective ngokusekelwe ezindleleni zesimanje zemithi (UHan et al., 2006). Kubuye kube yinto yokwelapha ebalulekile e-China futhi iyi-anti-inflammatory ethandwayo (Cha et al., 2009a),i-choleretic(U-Yoon noKim, ngo-2011kanye ne-anti-tumor (Feng et al., 2013)ikhambi lemithi.

    I-Phytochemicalucwaningo lwembule inani lamafutha abalulekile aguquguqukayo,ama-coumarins, futhii-flavonol ama-glycosideskanye neqembu elingaziwaama-aglyconeskusukaA. capillaris(Komiya et al., 1976,Yamahara et al., 1989). Amafutha abalulekile weA. capillaris(AEO) ingenye yezinhlanganisela ezisebenzayo zemithi futhi inikeza ukulwa nokuvuvukala (Cha et al., 2009a) kanye nezakhiwo ze-anti-apoptotic (Cha et al., 2009b). Kodwa-ke, njengoba i-AEO ingenye yezinhlanganisela eziyinhloko zeA. capillaris, imisebenzi engaba yi-hepatoprotective yezakhi ezinkulu ezivelaA. capillariskufanele ihlolwe.

    Kulolu cwaningo, umphumela wokuvikela we-AEO onI-carbon tetrachloride(CCl4) -inziwei-hepatotoxicityihlolwe ngezindlela ze-biochemical, njenge-hepatici-glutathione encishisiwe(GSH),malondialdehyde(MDA) amazinga,i-superoxide dismutase(SOD), kanyei-glutathione peroxidase(GSH-Px) umsebenzi, kanye nemisebenzi yei-aspartate aminotransferase(AST) kanyei-alanine aminotransferase(ALT) ku-serum. Izinga lokulimala kwesibindi okubangelwa i-CCl4 nalo lahlaziywa ngokubhekwa kwe-histopathological, kuhambisane nokuhlaziywa kwe-phytochemical okwenziwa yi-GC–MS ukuze kuhlonzwe izakhi ze-AEO.








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