Amafutha e-Artemisia capillaris ahlanzekile okwenza ikhandlela nensipho ngobuningi amafutha abalulekile amasha kuma-reed burner diffusers

Amafutha e-Artemisia capillaris ahlanzekile okwenza amakhandlela nensipho ngobuningi amafutha abalulekile amasha kuma-diffuser okushisa umhlanga Isithombe Esifakiwe

incazelo emfushane:

Umklamo wemodeli yegundane

Izilwane zahlukaniswa ngokungahleliwe zaba amaqembu amahlanu anamagundane ayishumi nanhlanu ngalinye. Iqembu lokulawula kanye namagundane eqembu lamamodeli anikezwe ithuba lokusebenzisauwoyela wesesameizinsuku eziyi-6. Amagundane eqembu lokulawula elihle ahlungwa ngamaphilisi e-bifendate (BT, 10 mg/kg) izinsuku eziyi-6. Amaqembu okuhlola aphathwa nge-100 mg/kg kanye ne-50 mg/kg AEO encibilikisiwe ku-sesame oil izinsuku eziyi-6. Ngosuku lwesi-6, iqembu lokulawula laphathwa nge-sesame oil, kanti wonke amanye amaqembu aphathwa ngedosi eyodwa ye-0.2% CCl4 ku-sesame oil (10 ml/kg) ngeumjovo wangaphakathi kwesisuAmagundane abe esezila ukudla ngaphandle kwamanzi, kwaqoqwa amasampula egazi emithanjeni ye-retrobulbar; igazi eliqoqiwe lafakwa ku-centrifuge ku-3000 ×gimizuzu eyi-10 ukuhlukanisa i-serum.Ukuhlukana komlomo wesibelethokwenziwa ngokushesha ngemva kokukhipha igazi, futhi amasampula esibindi asuswa ngokushesha. Ingxenye eyodwa yesampula yesibindi yagcinwa ngokushesha ku-−20 °C kuze kube yilapho kuhlaziywa, kanti enye ingxenye yasuswa futhi yagcinwa ku-10%i-formalinisixazululo; izicubu ezisele zigcinwe ku-−80 °C ukuze kuhlaziywe nge-histopathological (UWang nabanye, 2008,UHsu nabanye, 2009,UNie nabanye, 2015).

Ukulinganiswa kwamapharamitha e-biochemical ku-serum

Ukulimala kwesibindi kuhlolwe ngokulinganisaimisebenzi ye-enzymekwe-serum ALT kanye ne-AST kusetshenziswa ama-kit okuhweba ahambisanayo ngokwemiyalelo yama-kit (eNanjing, eSifundazweni saseJiangsu, eShayina). Imisebenzi ye-enzyme ivezwe njengamayunithi ngelitha (U/l).

Ukulinganiswa kwe-MDA, i-SOD, i-GSH kanye ne-GSH-Pxkuma-homogenates esibindini

Izicubu zesibindi zahlanganiswa nge-saline ebandayo yomzimba ngesilinganiso esingu-1:9 (w/v, liver:saline). Ama-homogenates ahlanganiswa nge-centrifuge (2500 ×gimizuzu eyi-10) ukuqoqa ama-supernatant ukuze kutholakale okulandelayo. Ukulimala kwesibindi kuhlolwe ngokwezilinganiso zesibindi zamazinga e-MDA kanye ne-GSH kanye ne-SOD kanye ne-GSH-Pximisebenzi. Konke lokhu kunqunywe ngokulandela imiyalelo esekhithini (eNanjing, eSifundazweni saseJiangsu, eShayina). Imiphumela ye-MDA kanye ne-GSH ivezwe njenge-nmol per mg protein (nmol/mg prot), kanye nemisebenzi ye-SOD kanye ne-GSH-P.xkwavezwa njenge-U per mg protein (U/mg protein).

Ukuhlaziywa kwe-Histopathological

Izingxenye zesibindi esisanda kutholwa zilungisiwe ku-10% bufferedi-paraformaldehydeisixazululo se-phosphate. Isampula yabe isifakwa ku-parafini, yasikwa yaba izingxenye ezingu-3-5 μm, yapendwa nge-i-hematoxylinfuthii-eosin(H&E) ngokwenqubo ejwayelekile, futhi ekugcineni ihlaziywe yi-i-microscopy ekhanyayo(UTian nabanye, 2012).

Ukuhlaziywa kwezibalo

Imiphumela ivezwe njenge-mean ± standard deviation (SD). Imiphumela ihlaziywe kusetshenziswa uhlelo lwezibalo i-SPSS Statistics, inguqulo 19.0. Idatha ihlolwe ukuhlukahluka (ANOVA,p< 0.05) kulandelwe ukuhlolwa kukaDunnett kanye nokuhlolwa kukaDunnett kwe-T3 ukuze kutholakale umehluko obalulekile ngokwezibalo phakathi kwamanani amaqembu ahlukahlukene okuhlola. Umehluko obalulekile ucatshangelwe ezingeni le-p< 0.05.

Imiphumela nengxoxo

Izakhi ze-AEO

Ngemva kokuhlaziywa kwe-GC/MS, i-AEO yatholakala iqukethe izakhi ezingu-25 ezishiywe imizuzu eyi-10 kuya kwengama-35, kwathi izakhi ezingu-21 eziqukethe u-84% wamafutha abalulekile zatholakala (Ithebula 1uwoyela oguquguqukayo oqukethweama-monoterpenoid(80.9%), ama-sesquiterpenoids (9.5%), ama-hydrocarbon agcwele amagatsha angagcwele (4.86%) kanye ne-acetylene ehlukahlukene (4.86%). Uma kuqhathaniswa nezinye izifundo (UGuo nabanye, 2004), sithole ama-monoterpenoid amaningi (80.90%) ku-AEO. Imiphumela ibonise ukuthi isakhi esitholakala kakhulu se-AEO yi-β-citronellol (16.23%). Ezinye izingxenye ezinkulu ze-AEO zifaka phakathi i-1,8-cineole (13.9%),i-camphor(12.59%),i-linalool(11.33%), α-pinene (7.21%), β-pinene (3.99%),i-thymol(3.22%), kanyei-myrcene(2.02%). Ukwehluka kokwakheka kwamakhemikhali kungahlobene nezimo zemvelo isitshalo esasivezwe kuzo, njengamanzi amaminerali, ukukhanya kwelanga, isigaba sokuthuthuka kanyeukudla okunomsoco.

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Sithumelele i-imeyili

Imininingwane Yomkhiqizo

Amathegi Omkhiqizo

Isifo sesibindi, isifo esivamile esibangelwai-hepatitis ebangelwa yigciwane, ukuphuza utshwala ngokweqile, amakhemikhali anobuthi esibindini, imikhuba yokudla engaphilile kanye nokungcola kwemvelo, kuyinto ekhathaza umhlaba wonke (UPapay nabanye, 2009). Kodwa-ke, ukwelashwa kwalesi sifo kuvame ukuba nzima ukukunikeza futhi kunomphumela olinganiselwe. IsiShayina sendabukoimithi yezitshalo, okuyiwona oyisisekelo semithi eminingi esetshenziswa ekwelapheni izifo zesibindi, isasetshenziswa kakhulu amaShayina (UZhao nabanye, 2014).i-Artemisia capillarisThumb.,I-AsteraceaeNgokusho kweBencao Gangmu, amarekhodi adumile kakhulu eMithi Yendabuko YaseShayina, asetshenziswe kabanzi njengomuthi wokususa ukushisa, ukukhuthazai-diuresisfuthi isuse i-jaundice futhi isetshenziswe njengokunambitheka eziphuzweni, imifino, kanye nama-pastries ngenxa yephunga layo elithile.A. i-capillarisibilokhu ibhekwa njengohlobo lomuthi wesintu waseShayina nokudla ngabantu abaningi. Ngakho-ke, kube nemizamo emikhulu yokuthuthukisa imithi yamakhambi ewusizo, njengeA. i-capillaris, yokwelapha isifo sesibindi.

Eminyakeni yamuva nje, imithi yamakhambi ithole ukunakwa okukhulu nokuthandwa kakhulu ekwelapheni izifo zesibindi ngenxa yokuphepha nokusebenza kwayo (UDing nabanye., 2012).A. i-capillariskuye kwafakazelwa ukuthi kunomsebenzi omuhle wokuvikela isibindi ngokusekelwe ezindleleni zesimanje zemithi (UHan nabanye., 2006Futhi iyinto ebalulekile yokwelapha eShayina futhi iyindlela ethandwayo yokulwa nokuvuvukala (UCha nabanye, 2009a),i-choleretic(UYoon noKim, 2011), kanye nokulwa nomdlavuza (UFeng nabanye, 2013)umuthi wezitshalo.

Amakhemikhali e-phytochemicalIzifundo ziveze inani lamafutha abalulekile aguquguqukayo,ama-coumarin, futhii-flavonol ama-glycosidekanye neqembu labantu abangaziwaama-aglyconeskusukelaA. i-capillaris(UKomiya nabanye, 1976,UYamara nabanye, 1989Amafutha abalulekile e-A. i-capillaris(AEO) ingenye yamakhemikhali asebenzayo kwezokwelapha futhi inikeza ukulwa nokuvuvukala (UCha nabanye, 2009a) kanye nezakhiwo zokulwa nokuvuvukala kwezicubu (UCha nabanye, 2009bKodwa-ke, njengoba i-AEO ingenye yamakhemikhali ayinhlokoA. i-capillaris, imisebenzi engaba khona yokuvikela isibindi yezingxenye ezinkulu ezivelaA. i-capillariskufanele kuhlolwe.

Kulolu cwaningo, umphumela wokuvikela we-AEO kui-tetrachloride yekhabhoniokubangelwa yi-(CCl4)ubuthi besibindikuhlolwe ngezindlela ze-biochemical, njenge-hepatici-glutathione encishisiwe(GSH),i-malondialdehydeamazinga (MDA),i-superoxide dismutase(i-SOD), kanyei-glutathione peroxidase(GSH-P)x) umsebenzi, kanye nemisebenzi yei-aspartate aminotransferase(AST) kanyei-alanine aminotransferase(ALT) ku-serum. Ubukhulu bokulimala kwesibindi okubangelwa yi-CCl4 buphinde bahlaziywa ngokubhekwa kwe-histopathological, okuhambisana nokuhlaziywa kwe-phytochemical yi-GC-MS ukuze kutholakale izakhi ze-AEO.